CHROMOSOME ANOMALIES DO NOT INCREASE IN RESCUE-IVM OOCYTES: ANALYSIS OF TROPHECTODERM BIOPSIES BY NEXT-GENERATION SEQUENCING FOR PREIMPLANTATION GENETIC TESTING

The in vitro maturation of GV oocytes retrieved from gonadotrophin-stimulated IVF cycles (rescue-IVM) is generally not recommended because the lower developmental competence, implantation rate, and higher rate chromosomal anomalies than vivo-matured oocytes. However, most relevant studies analyzed cleavage stage embryos may therefore have included some that were developmentally arrested. Additionally, few examined rescue-IVM mild stimulation such as clomiphene cycle letrozole cycle. We 41 blastocysts 8 patients under or March to December 2020. For all patients, obtained both vitro- Mean patient age was 35.5 ± 5.3 years (range 26 years). Blastocysts derived classified (n = 9) control 32) groups, respectively. n group, cultured for 27 hours IVM medium matured used ICSI. In MII subjected Prospective zygotes a time-lapse incubator (EmbryoScope+) day 0 trophectoderm biopsy. Trophectoderm biopsy performed at 5 6 next-generation sequencing analyze numerical chromosome embryos. rates aneuploidy groups 44.4% (4/9) 50.0% (16/32), There no significant difference between groups. One euploid embryo only rescue-IVM. show similar chromosomally normal